Design of an enantioselective artificial metallo-hydratase enzyme containing an unnatural metal-binding amino acid

Design of an enantioselective artificial metallo-hydratase enzyme containing an unnatural metal-binding amino acid.

The design of artificial metalloenzymes is a challenging, yet ultimately highly rewarding objective because of the potential for accessing new-to-nature reactions. One of the main challenges is identifying catalytically active substrate-metal cofactor-host geometries. The advent of expanded genetic code methods for the in vivo incorporation of non-canonical metal-binding amino acids into protei...

Design of an enantioselective artificial metallo-hydratase enzyme containing an unnatural metal-binding amino acid† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc03477f Click here for additional data file.

Molecular dynamics simulations of peptides containing an unnatural amino acid: dimerization, folding, and protein binding.

We have performed molecular dynamics (MD) simulations to study the dimerization, folding, and binding to a protein of peptides containing an unnatural amino acid. NMR studies have shown that the substitution of one residue in a tripeptide beta-strand by the unnatural amino acid Hao (5-HO2CCONH-2-MeO-C6H3-CO-NHNH2) modifies the conformational flexibility of the beta-strand and the hydrogen-bondi...

An unnatural amino acid that mimics phosphotyrosine.

By replacing the phosphate group in pTyr with an isoxazole carboxylic acid, a novel unnatural amino acid has been successfully synthesized. Subsequently, its incorporation into a known PPI (protein-protein interaction) inhibitor of STAT3 protein (ISS 610) generated I2 which showed reasonable anti-STAT3 activity in both fluorescence polarization and cell-proliferation experiments.

design of an analog fuzzy logic controller chip

fuzzy logic has been developed over the past three decades into a widely applied techinque in classification and control engineering. today fuzzy logic control is one of the most important applications of fuzzy set theory and specially fuzzy logic. there are two general approachs for using of fuzzy control, software and hardware. integrated circuits as a solution for hardware realization are us...